ProductCategory: Pre-maderecombinantAAV;AAV-CAG-FLPo;AAV-CBA-FLPo;AAV6-CAG-FLPo;AAV6-CBA-FLPo
Description: FLPoriginallyidentifiedfromSaccharomycescerevisiaeisasite-specificrecombinases(SSR)fromtheφintegrasefamilyandrecognizesdistinct34bpFRTsequence.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem.InitialuseofFLPinmammaliancellsrevealedinefficientrecombinaseactivityduetoThermo-instABIlityoftheFLPprotein.Subsequentscreeningforthermo-stablemutantsresultedintheidentificationofFLPewhichwas4-foldincreaseinrecombinationefficiencyat37°C.HowevertherecombinationefficiencyofFLPeincellsremainsverylowbecauseofitsnon-mammalianorigin.
FLPoisamousecodonoptimizedFLPethatgreatlyincreasestheFRTrecombinationefficiencyforbothinvivoandinvitroinmouse. ThisAAV6-CAG-FLPopre-made rAAVinSEROtype6(withcapsidfromAAVserotype6and2xITRfromAAVserotype2)will over-expressFLPorecombinaseunderCAGpromoter(alsoknownasCBApromoter). Readytouseformat.
Titer: >1x10^13 VG/mL
Size: > 30 µL(StdPack)
StorageBuffer: SterilePBS
InfectionProtocol:
VectorMap: